Prevalence of Salmonella Pathogenisity Island (SPI1, SPI2, SPI3 and SPI5) Genes in Salmonella species Isolated from fresh Broiler Chicken Meat in Sri Lanka

Purpose: Salmonella is a significant pathogen affecting wider range of animals and one the main causes diarroeal diseases leading to millions human cases globally. Pathogenicity islands (SPI) are imperative in invasion host cell pathogenesis within cell. It found that SPI from 1 5 present every serovars S. enterica these laterally acquired virulence regions.Research Method: This study investigated presence some genes belong SPI1, SPI2, SPI3 SPI5 23 isolates broiler chicken meat Sri Lanka using PCR method further (gene encoding invasion-associated protein, structural gene for attachment invasion, regulatory like nucleoid structuring gene) were quantified RT- PCR.Findings: The revealed Island such as hilA, invH, invF, invA hns all isolates. Among 2 investigated, showed ssaO, ssaQ, ssaP ssaS (genes T3SS apparatus proteins), sscB sscA secretion system chaperon sseF, sseD coding secreted eff ector proteins). In isolates, 3 genes, responsible magenisum transport (mgtB mgtC) found. rhuM (that supposed code cytoplasmic protein) was absent four whereas cigR (which predicted codes membrane two pipB except isolate sopB isolate.Originality/Value: conserved most fresh expression diff erent vary with isolate. molecular basic will pave way explore more on local Salmonella, detection methods control methods.